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Regulatory

Part:BBa_K950000:Design

Designed by: Jakob Matthes   Group: iGEM12_Tuebingen   (2012-07-10)

yeast fet3 promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

length of promoter was chosen to include binding sites and to obtain good primers


Source

Genomic upstream sequence of fet3 gene from the [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=fet3 Saccharomyces Genome Database, FET3].

References

  1. [http://www.ncbi.nlm.nih.gov/pubmed/8293473 ASKWITH, C., EIDE, D., VAN HO, A., BERNARD, P. S., LI, L., DAVIS-KAPLAN, S., SIPE, D. M. & KAPLAN, J. 1994. The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake. Cell, 76, 403-410.]
  2. [http://www.ncbi.nlm.nih.gov/pubmed/17553578 KUPCHAK, B. R., GARITAONANDIA, I., VILLA, N. Y., MULLEN, M. B., WEAVER, M. G., REGALLA, L. M., KENDALL, E. A. & LYONS, T. J. 2007. Probing the mechanism of FET3 repression by Izh2p overexpression. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1773, 1124-1132.]
  3. [http://www.ncbi.nlm.nih.gov/pubmed/15060275 LYONS, T. J., VILLA, N. Y., REGALLA, L. M., KUPCHAK, B. R., VAGSTAD, A. & EIDE, D. J. 2004. Metalloregulation of yeast membrane steroid receptor homologs. Proceedings of the National Academy of Sciences of the United States of America, 101, 5506-5511.]
  4. [http://www.ncbi.nlm.nih.gov/pubmed/18603275 SMITH, J. L., KUPCHAK, B. R., GARITAONANDIA, I., HOANG, L. K., MAINA, A. S., REGALLA, L. M. & LYONS, T. J. 2008. Heterologous expression of human mPRα, mPRβ and mPRγ in yeast confirms their ability to function as membrane progesterone receptors. Steroids, 73, 1160-1173.]